Composition and Action of Yeast Polygalacturonase
نویسندگان
چکیده
منابع مشابه
The unienzymatic nature of yeast polygalacturonase.
Yeast polygalacturonase (YPG) catalyzes the hydrolysis of pectic acid to a mixture of digalacturonic and galacturonic acids (1). The reaction course consists of (a) an initial rapid attack on the linear chain by a random mechanism until about 25 per cent hydrolysis has occurred, (b) a slower phase during which an additional 25 per cent of the available bonds are broken, and (c) an extremely slo...
متن کاملHydrolysis of the oligogalacturonides and pectic acid by yeast polygalacturonase.
Yeast polygalacturonase (YPG) is a constitutive type enzyme, produced exocellularly and free of pectinesterase by Saccharomyces jragilis (1). It has an optimal pH of 4.4 and appears to be specific in its activity towards polygalacturonides (2). YPG differs from preparations of commercial fungus polygalacturonase in that it cannot carry out a complete breakdown of pectic acid to galacturonic aci...
متن کاملOn the mechanism of action of yeast endo-polygalacturonase on oligogalacturonides and their reduced and oxidized derivatives.
Yeast polygalacturonase catalyzes a random hydrolysis of pectic acid to a mixture of digalacturonic and galacturonic acids via a series of oligogalacturonides (1). This enzyme will be termed yeast endo-polygalacturonase to differentiate it from end group-hydrolyzing enzymes, which have been named exopolygalacturonases (2). Evidence has been presented that yeast endo-polygalacturonase is a singl...
متن کاملIsolation and Characterization of Polygalacturonase Produced by Tetracoccosporium sp.
Thirty-five fungal strains which isolated from vegetable wastes, were screened for the use of polygalacturonic acid as the sole carbon source. Twenty-five isolates were positive for polygalacturonase activity in cup-plate assay, as evidenced by clear hydrolysation zones. The most productive strain was determined by measuring clear zones formed around colonies stained with ruthenium red.</em...
متن کاملIsolation of a yeast strain able to produce a polygalacturonase with maceration activity of cassava roots
Received 11/9/2012 Accepted 7/3/2013 (005877) 1 Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Felix de Azara, 1552, CP 3300, Posadas, Misiones, Argentina, e-mail: [email protected] 2 Instituto Nacional de Tecnología Agropecuaria – INTA, EEA Mendoza, San Martín, 3853, CP 5507, Luján de Cuyo, Mendoza, Argentina 3 Universidad Nacional del Chaco Aust...
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ژورنال
عنوان ژورنال: Nature
سال: 1954
ISSN: 0028-0836,1476-4687
DOI: 10.1038/174515a0